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Image Search Results
Journal: Arthritis research & therapy
Article Title: Circ-CAMTA1 regulated by Ca 2+ influx inhibited pyruvate carboxylase activity and modulate T cell function in patients with systemic lupus erythematosus.
doi: 10.1186/s13075-024-03422-6
Figure Lengend Snippet: Fig. 2 Differential expression of circRNA in Jurkat cells after coculture with ionomycin (1 µg/mL) for 1 h. (A) The expression profiles of circRNA in Jurkat cells after coculture with ionomycin (1 µg/mL) for 1 h were evaluated using next-generation sequencing. Each scatter point in the figure represents the mean read count of circRNA in three replicates of the treatment. (B) Validation of potential candidates of Ca2+ influx-regulated circRNAs in Jurkat cells. The relative expression level of circRNA was defined as (39 - Ct) after adjusted with the internal control (GAPDH; Glyceraldehyde-3-Phosphate Dehydro genase). Data was presented as mean ± SD. (ns, not significant; *P < 0.05; **P < 0.01; ***P < 0.001)
Article Snippet: To analyzed circRNA expression levels in Jurkat cells after stimulation, cells were treated with phorbol 12-myristate 13-acetate (PMA; 20 ng/mL; Sigma-Aldrich) and ionomycin (500 ng/mL; Sigma-Aldrich, ) for 24 h. For analysis of
Techniques: Quantitative Proteomics, Expressing, Next-Generation Sequencing, Biomarker Discovery, Control